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KMID : 0613820100200121872
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Journal of Life Science
2010 Volume.20 No. 12 p.1872 ~ p.1881
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Induction of Apoptosis by Ethanol Extracts of Fermented Agabeans in AGS Human Gastric Carcinoma Cells
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Kim Sung-Ryeal
Lee Hye-Hyeon
Kong Tae-Young
Seo Min-Jeong
Hong Su-Hyun
Choi Yung-Hyun
Kang Byoung-Won
Park Jeong-Uck
Joo Woo-Hong
Rhu Eun-Ju
Jeong Yong-Kee
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Abstract
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Extracts of soybeans fermented by Bacillus subtilis have a wide variety of functions, such as enhancing the body"s immune function, fibrinolysis activity, anti-inflammation, anti-cancer, estrogen function and anti-infection effects. Recently, it was reported that the extracts of fermented beans exhibit strong anti- inflammatory and anti-cancer properties by suppressing the transcription of pro-inflammatory cytokine genes and induction of apoptosis, respectively. However, the mechanisms of their cytotoxicity in human gastric cancer cells are poorly understood. In the present study, we investigated the effects of ethyl alcohol extracts from fermented soybean (FS) and yellow agabean (FYA) on cell growth and apoptosis in AGS human gastric cancer cells. A treatment of FS and FYA inhibited the growth of AGS cells in a concentration-dependent manner by inducing apoptosis. FS- and FYA-induced apoptosis were associated with down-regulation of XIAP and cIAP-2, and up-regulation of pro-apoptotic Bax expression. Moreover, a treatment of FS and FYA not only triggered an increase in the levels of death receptor (DR)4, DR5, Fas and FasL, but also induced the activation of casepase-3, -8 and -9. These findings illustrate that FS and FYA may have a therapeutic potential in human gastric AGS cells and as a functional food.
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KEYWORD
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AGS, apoptosis, fermented soybeans
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